Comparison of the Prosky Method and the Fiber Tester Dietary fiber refers to the sum of edible plant cells, polysaccharides, lignin, and related substances that cannot be digested and absorbed by human endogenous enzymes. Dietary fiber has attracted the attention of nutritionists in various countries because of its strong oil holding capacity, water holding capacity, and solubilization and induction of microorganisms. China has a large number of peanuts, 50% of which are used for crushing oil, and peanuts are the product of peanuts after crushing oil. China's peanut bran is mainly used as animal feed and plant fertilizer. Fiber content of peanut bran is determined by using a crude fiber analyzer. After the measurement, there is a great relationship among the quality of animals among the feeds used for animals. The determination of fiber content in peanut bran can also be measured using the Prosky method. Here is a brief look at the gap between the two methods:
Prosky's method of operation is: first accurately weighed 3.000 0 g of defatted sample, under the optimal conditions of enzymatic hydrolysis, enzymatic hydrolysis with amylases, and then continue to use glucoamylase to further enzymatic hydrolysis products of oligosaccharides and dextrin enzyme The solution is glucose, and then trypsin digestion to remove the protein in the sample, the enzymolysis solution is filtered, the filtrate is precipitated with 4 volumes of absolute ethanol, allowed to stand for 2 h, filtered, and dried to obtain SDF; the filter residue is washed and dried. The measured value can be obtained.
The procedure for using the crude fiber analyzer is to digest the sample according to the currently used acid-alkali digestion method, and to determine the crude fiber content of the sample by weight measurement. The crude fiber analyzer uses accurate concentrations of acids and bases to deconcoct the sample under specific conditions, and then removes soluble substances with ethanol. After burning at high temperatures, the amount of minerals is deducted. The crude fiber content is called crude fiber. The measurement accuracy of this method of operation is in line with the requirements of national standards. It is a relatively good measurement method. A single coarse fiber analyzer can be used to measure six samples at the same time.
The measurement of fiber content in various foods is important because it has no nutritional value, but it is closely related to human nutrition and disease, and can prevent and treat various diseases such as rectal cancer, diabetes, high blood pressure, Appendicitis, acne, etc. are called "seventh nutrients" after starch, protein, fat, vitamins, minerals, and water.
Prosky's method of operation is: first accurately weighed 3.000 0 g of defatted sample, under the optimal conditions of enzymatic hydrolysis, enzymatic hydrolysis with amylases, and then continue to use glucoamylase to further enzymatic hydrolysis products of oligosaccharides and dextrin enzyme The solution is glucose, and then trypsin digestion to remove the protein in the sample, the enzymolysis solution is filtered, the filtrate is precipitated with 4 volumes of absolute ethanol, allowed to stand for 2 h, filtered, and dried to obtain SDF; the filter residue is washed and dried. The measured value can be obtained.
The procedure for using the crude fiber analyzer is to digest the sample according to the currently used acid-alkali digestion method, and to determine the crude fiber content of the sample by weight measurement. The crude fiber analyzer uses accurate concentrations of acids and bases to deconcoct the sample under specific conditions, and then removes soluble substances with ethanol. After burning at high temperatures, the amount of minerals is deducted. The crude fiber content is called crude fiber. The measurement accuracy of this method of operation is in line with the requirements of national standards. It is a relatively good measurement method. A single coarse fiber analyzer can be used to measure six samples at the same time.
The measurement of fiber content in various foods is important because it has no nutritional value, but it is closely related to human nutrition and disease, and can prevent and treat various diseases such as rectal cancer, diabetes, high blood pressure, Appendicitis, acne, etc. are called "seventh nutrients" after starch, protein, fat, vitamins, minerals, and water.
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