The robust grape plants with no pests and diseases were selected and the representative 1a branches in the middle and upper parts were cut. Rinse with tap water first, then rinse with 3~5 times of ion-free water, wrap with plastic bag and clean wet gauze, and store at 0 °C refrigerator for use. The varieties are: Ai Bo Xin No. 1, Red Milk, Kyoho, and Beauty. Instrument: conductivity meter, electric suction device, horizontal low-temperature freezer, intelligent light incubator (25 °C, 80% of the illumination), one-tenth electronic balance, electric blast drying oven and so on.
The gauze wrapped branches were placed in 0, -5, -10, -15, -20, -25, -30, -35°C 8 treated low-temperature refrigerators for 24h, then 5°C/h After the temperature was raised to 0°C, the shoots were taken out and thawed at room temperature for 1 hour. The water was blotted dry with filter paper. The frozen shoots were cut into 0.5 cm sections (without buds). 3 g of the shoots were placed in a test tube and 30 mL of ion-free water was added. Vacuum infiltration 10min, standing about 12h. Conductivity meter was used to measure the electrolyte exudation rate of the extract; then the sample was sealed and placed in a 98°C oven for 1 h, and the electrolyte exudation rate of the extract was determined after standing for about 12 h, which was the complete destruction of the cells. Electrolyte exudation rate to represent the total amount of electrolytes in vitro shoot cells. The electrolyte exudation rate under natural conditions (0°C) was used as a control, and the other measurement procedures were the same.
With the decrease of the treatment temperature of the intelligent light incubator, the degree of injury was aggravated, and the relative conductivities of the grape varieties became larger and larger, showing an increasing trend, and the damage of the cell membrane was aggravated by the low temperature treatment. Therefore, according to different low-temperature treatments and their conductivity values, the order of cold resistance of these four grape varieties was: Aiboxin No. 1> Kyoho> Hongkui> Meirenzhi. Plants under low temperature stress, the semipermeable membrane of the cell membrane damage by extracellular electrolyte leakage damage increased, and the different levels of cell damage in different plants with different electrolyte leakage changes, so the electrolyte leakage rate can be used to reflect the degree of tissue damage And the size of plant cell resistance, the relative conductivity before and after low-temperature injury is used to represent the permeability of the electrolyte in the membrane.
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